老司机午夜精品99久久免费,国产色无码专区在线观看,动漫人物桶动漫人物免费观看网站,99精产国品一二三产区

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>CRL-2888WPE-int 人正常前列腺上皮細(xì)胞

WPE-int 人正常前列腺上皮細(xì)胞

簡要描述:CRL-2888 WPE-int 人正常前列腺上皮細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種原代細(xì)胞、細(xì)胞系。細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號:CRL-2888
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-16
  • 訪  問  量:1440

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-2888 WPE-int 人正常前列腺上皮細(xì)胞

ATCC® Number:CRL-2888™    Price:$329.00
Designations:WPE-int

Depositors:MM Webber, EJ Tokar

Biosafety Level:2 [cells contain human HPV-18 viral DNA sequences ]

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:epithelial
WPE-int 人正常前列腺上皮細(xì)胞


Source:Organ: prostate, normal, peripheral zone
Cell Type: epithelial


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-2888 WPE-int 人正常前列腺上皮細(xì)胞

Isolation:Isolation date: 2004 [PubMed: 16351690] [92794]

Receptors:androgen receptor, expressed ( [92794] upregulated upon exposure to androgen)

Tumorigenic:NO

Antigen Expression:kallikrein 3 ( KLK3); prostate specific antigen (PSA); expressed (upregulated upon exposure to androgen) [PubMed: 16351690] [92794]

DNA Profile (STR):Amelogenin: X,Y

CSF1PO: 13

D13S317: 8,14

D16S539: 9,11

D5S818: 12,15

D7S820: 10,11

THO1: 8

TPOX: 8,11

vWA: 14,18



Age:54

Gender:male

Ethnicity:Caucasian

Comments:WPE-int cells were derived from the RWPE-1 cell line (ATCC CRL-11609) after two consecutive cycles of single cell cloning. To establish the RWPE-1 cell line, epithelial cells from the peripheral zone of a histologically normal adult human prostate were transfected with a plasmid carrying one copy of the human papilloma virus 18 (HPV-18) genome. WPE-int cells exhibit features characteristic of an intermediate cell type on the path to luminal cell differentiation. Cells show very low expression of p63 and MMP-2 and high expression of cytokeratin 18 as compared to the WPE-stem cell line (ATCC CRL-2887). They are anchorage-dependent. [PubMed: 16351690]The WPE-stem cell line (ATCC CRL-2887), which expresses several features characteristic of stem/progenitor cells, was also derived from RWPE-1 cells after single cell cloning.
  1. The parental cell line RWPE-1 was screened for CMV, HBV, HCV, HTLV 1, HTLV 2, HIV 1, HIV 2, JCV, and MoMuLV DNA sequences. Cells were also tested for 25 species of mycoplasma and Acholeplasma laidlawii . Cells tested negative for all of the above. (personal communication from depositor).



Propagation:ATCC complete growth medium: The base medium for this cell line is provided by Invitrogen (GIBCO) as part of a kit: Keratinocyte Serum Free Medium (K-SFM), Kit Catalog Number 17005-042. This kit is supplied with each of the two additives required to grow this cell line (bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF). To make the complete growth medium, you will need to add the following components to the base medium:
  • 0.05 mg/ml BPE - provided with the K-SFM kit

  • 5 ng/ml EGF - provided with the K-SFM kit. NOTE: Do not filter complete medium.
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37.0°C
    Growth Conditions: Subculture cells before they reach confluence. Do not allow cells to become confluent.




CRL-2888

ATCC® Number: CRL-2888™ Price: $329.00
Designations: WPE-int
Depositors: MM Webber, EJ Tokar
Biosafety Level: 2 [cells contain human HPV-18 viral DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: epithelial


Source: Organ: prostate, normal, peripheral zone
Cell Type: epithelial
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: 2004 [PubMed: 16351690] [92794]
Receptors: androgen receptor, expressed ( [92794] upregulated upon exposure to androgen)
Tumorigenic: NO
Antigen Expression: kallikrein 3 ( KLK3); prostate specific antigen (PSA); expressed (upregulated upon exposure to androgen) [PubMed: 16351690] [92794]
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 13
D13S317: 8,14
D16S539: 9,11
D5S818: 12,15
D7S820: 10,11
THO1: 8
TPOX: 8,11
vWA: 14,18
Age: 54
Gender: male
Ethnicity: Caucasian
Comments: WPE-int cells were derived from the RWPE-1 cell line (ATCC CRL-11609) after two consecutive cycles of single cell cloning. To establish the RWPE-1 cell line, epithelial cells from the peripheral zone of a histologically normal adult human prostate were transfected with a plasmid carrying one copy of the human papilloma virus 18 (HPV-18) genome. WPE-int cells exhibit features characteristic of an intermediate cell type on the path to luminal cell differentiation. Cells show very low expression of p63 and MMP-2 and high expression of cytokeratin 18 as compared to the WPE-stem cell line (ATCC CRL-2887). They are anchorage-dependent. [PubMed: 16351690]The WPE-stem cell line (ATCC CRL-2887), which expresses several features characteristic of stem/progenitor cells, was also derived from RWPE-1 cells after single cell cloning.
The parental cell line RWPE-1 was screened for CMV, HBV, HCV, HTLV 1, HTLV 2, HIV 1, HIV 2, JCV, and MoMuLV DNA sequences. Cells were also tested for 25 species of mycoplasma and Acholeplasma laidlawii . Cells tested negative for all of the above. (personal communication from depositor).
Propagation: ATCC complete growth medium: The base medium for this cell line is provided by Invitrogen (GIBCO) as part of a kit: Keratinocyte Serum Free Medium (K-SFM), Kit Catalog Number 17005-042. This kit is supplied with each of the two additives required to grow this cell line (bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF). To make the complete growth medium, you will need to add the following components to the base medium:
•0.05 mg/ml BPE - provided with the K-SFM kit
•5 ng/ml EGF - provided with the K-SFM kit. NOTE: Do not filter complete medium.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Growth Conditions: Subculture cells before they reach confluence. Do not allow cells to become confluent.



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
天堂8中文在线最新版官网| av免费观看| 影音先锋资源站| 中文日文字幕视频| 波多野结衣办公室性xxx| 漂亮的保姆3在线看| 男人呻吟双腿大开男男h漫画在线| 亚洲国产激情一区二区三区| 一边亲着面膜胸口| 国产视频在线观看| 波多野结衣av无码| bt樱桃 磁力岛| 国产综合精品| 教官脔到她哭h粗话h好爽视频| 猛烈顶弄h禁欲老师h春潮| 光棍天堂手机2019版免费观看| 人妻无码中文字幕| chinese粗口篮球体育生生| 欧美性情免费观看| 免费无码又爽又刺激高潮的app| 大明荫蒂女人毛茸茸| 无码人妻一区二区三区免费视频| 男女边吃奶边做边爱视频| 国产乱来乱子视频| 一女侍七夫巨h双修| 办公室双腿打开揉弄高潮女明星| 国精产品一区一区三区有限在线| 无码熟熟妇丰满人妻啪啪软件| 在线永久免费观看黄网站| 777久久精品一区二区三区无码| 亚洲A∨无码一区二区三区| 岛国无码AV不卡一区二区| 久精品国产欧美亚洲色AⅤ大片| 玩弄japan白嫩少妇hd小说| 业余 自由 性别 成熟偷窥 | 老师你下面太紧了拔不出来| 少妇人妻av毛片在线看| 善良的小峓子hd完整视频中文| 强奷人妻日本中文字幕| 国产精品成人久久久久久久| 色欲无码国产喷水av精品|