老司机午夜精品99久久免费,国产色无码专区在线观看,动漫人物桶动漫人物免费观看网站,99精产国品一二三产区

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>CCL-171MRC-5 人胚肺細(xì)胞

MRC-5 人胚肺細(xì)胞

簡要描述:CCL-171 MRC-5 人胚肺細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號:CCL-171
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-16
  • 訪  問  量:2133

產(chǎn)品分類

Product Category

詳細(xì)介紹

CCL-171 MRC-5 人胚肺細(xì)胞

ATCC® Number:  CCL-171™

Designations:  MRC-5

Depositors:   JP Jacobs

Biosafety Level: 1

Shipped:  frozen

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: fibroblast

CCL-171 MRC-5 人胚肺細(xì)胞

Source: Organ: lung

Disease: normal

Cell Type: fibroblast

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Isolation:  Isolation date: September, 1966

Applications: efficacy testing [92587]

testing [92346] [92389]

transfection host (Roche FuGENE® Transfection Reagents)

viruscide testing [92579] [92582]

Virus Susceptibility: poliovirus 1; herpes simplex; vesicular stomatitis (Indiana)

Reverse Transcript: negative

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 11,12

D13S317: 11,14

D16S539: 9,11

D5S818: 11,12

D7S820: 10,11

THO1: 8

TPOX: 8

vWA: 15

Cytogenetic Analysis: Chromosome Frequency Distribution 50 Cells: 2n = 46. This is a normal diploid human cell line with 46,XY karyotype. The modal chromosome number was 46, occurring in 70% of cells. The rate of polyploidy was 3.6%. Both X and Y chromosomes were normal.

Isoenzymes:  G6PD, B

Age:  14 weeks gestation

Gender:  male

Ethnicity:  Caucasian


Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing:  Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.

CCL-171



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
亚洲欧美日韩一区二区| 亚洲人成无码网站在线观看 | 国产chinese男男gay片| 男人和女人高潮做爰视频| 国产一区二区三区在线观看| 永久免费40分钟看大片| 黑人巨大JEEP日本人| 欧美日韩亚洲国产精品| 少妇性l交大片免费| 久久精品国产一区二区三区| 福彩3d字谜图谜总汇| 亚洲中文字幕无码久久2021| 真实单亲乱l仑对白视频| 小13箩莉黄瓜自慰喷白浆| 亚洲国产成人精品无码区99 | 波多野结AV衣东京热无码专区 | 麻豆亚洲av成人无码久久精品 | 久久日本片精品AAAAA国产| 奴役 支配 性狂虐 电击 极端 | gogo全球大胆高清人体444| 我和两个小婕子做过爱| 国产做爰又粗又大免费看| 狠天天狠天天香蕉网| 国产a级毛片| 国产大学生粉嫩无套流白浆| 多人交free性glasses| 久久久久久综合网天天| 日本人妻a片成人免费看| 色狠狠AV老熟女| 中文在线中文资源| 最近2019中文字幕大全视频1| 色戒2小时38分无删减版| 母亲6韩国电视剧免费观看| 浪漫樱花动漫在线观看免费| 人人妻人人澡人人爽超污| 午夜福利免视频100集2019| 人人妻人人澡人人爽国产| 男男开荤粗肉np尿在里面| 邻居的丰满人妻hd学生| 免费a级毛片无码专区| 又长又粗又大又硬起来了|