老司机午夜精品99久久免费,国产色无码专区在线观看,动漫人物桶动漫人物免费观看网站,99精产国品一二三产区

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>IMR-90人胚肺成纖維細(xì)胞

人胚肺成纖維細(xì)胞

簡要描述:CCL-186 IMR-90 人胚肺成纖維細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號:IMR-90
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-12
  • 訪  問  量:2644

產(chǎn)品分類

Product Category

詳細(xì)介紹

CCL-186 IMR-90人胚肺成纖維細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

CCL-186 IMR-90人胚肺成纖維細(xì)胞.

ATCC® Number: CCL-186™

Designations: IMR-90

Depositors: WW Nichols

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: fibroblast

CCL-186 IMR-90人胚肺成纖維細(xì)胞.

Source: Organ: lung

Disease: normal

Cell Type: fibroblast

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Applications: transfection host (Nucleofection technology from Lonza)

Virus Susceptibility: Human poliovirus 1

Human poliovirus 2

Varicella-Zoster

Herpes simplex virus 1

Herpes simplex virus 2

Human poliovirus 3

Vaccinia virus

Human herpesvirus 5

Vesicular stomatitis virus

Reverse Transcript: negative

DNA Profile (STR): Amelogenin: X

CSF1PO: 11,13

D13S317: 11,13

D16S539: 10,13

D5S818: 12,13

D7S820: 9,12

THO1: 8,9.3

TPOX: 8,9

vWA: 16,19

Cytogenetic Analysis: normal human female; diploid; stable

Isoenzymes: G6PD, B

Age: 16 weeks gestation

Gender: female

Ethnicity: Caucasian

Comments: The human diploid fibroblast strain IMR-90 was derived by W.W. Nichols and associates from the lungs of a 16-week female fetus. [22381]

The division potential, viral susceptibilities and other properties have been thoroughly studied such that the line may be considered as an alternate for WI-38 and other standard human lung cell strains.

The cells have been reported to be capable of attaining 58 population doublings before the onset of senescence. [22381]

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:8 is recommended

Medium Renewal: Every 3 to 4 days

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

References: 22381: Nichols WW, et al. Characterization of a new human diploid cell strain, IMR-90. Science 196: 60-63, 1977. PubMed: 841339

32932: Dolganov GM, et al. Human Rad50 is physically associated with human Mre11: identification of a conserved multiprotein complex implicated in recombinational DNA repair. Mol. Cell. Biol. 16: 4832-4841, 1996. PubMed: 8756642

33041: Ostlund RE Jr., et al. A sterospecific myo-inositol/D-chiro-inositol transporter in HepG2 liver cells. J. Biol. Chem. 271: 10073-10078, 1996. PubMed: 8626564

Related Links

NCBI Entrez Search

Make a Deposit

Frequently Asked Questions

Material Transfer Agreement

Technical Support

Related Cell Culture Products


IMR-90人胚肺成纖維細(xì)胞更多信息,請致電咨詢。


















 

產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
脱了美女内裤猛烈进入GIF| 日韩毛片无码永久免费看| 欧美白人最猛性XXXXX| 9277在线观看免费播放| 性色少妇AV蜜臀人妻无码| 八戒八戒神马在线电影免费| 国产精品国产三级国产AV中文| 97av麻豆蜜桃一区二区| 国产精品18久久久久久不卡| 一本色道久久综合亚洲精品| 啦啦啦资源视频在线观看8| 朋友出差人妻半推半就滑进| 日本三级片在线观看| 欧美丰满熟妇多毛XXXXX| 扶着人妻翘臀挺进| 刚开始一深一浅后来逐渐变大 | 国产女人的高潮国语对白入口| 亚洲av无一区二区三区久久| 免费a级毛片无码视频| 免费无码一区二区三区蜜桃| 影音先锋女人av鲁色资源网| 中文字幕人妻女友一区蜜芽视频 | 啦啦啦在线视频免费观看| 一边啪啪一边呻吟av夜夜嗨| 国产jizzjizz麻豆全部免费| 日产精品高潮呻吟av久久| 在线bt天堂网www在线| 妺妺窝人体色www看美女| 熟女chachacha性少妇| 乱LUN合集1第40部分阅读| 人妻巨大乳挤奶水HD免费看| 真人做爰45分钟| 欧美色综合天天久久综合精品| 偷拍真实夫妇作爱视频 | 成人性生交大片免费看一| 日韩蜜桃一区二区三区| 女局长白白嫩嫩大屁股| 国产精品久久人妻无码网站仙踪林 | 狠狠挺进稚嫩学生小身体| 老师把腿翘起来我要进去了| 特种兵的又粗又大好爽H|