老司机午夜精品99久久免费,国产色无码专区在线观看,动漫人物桶动漫人物免费观看网站,99精产国品一二三产区

咨詢熱線

15000266580

當前位置:首頁 >產(chǎn)品中心>細胞庫>人腫瘤細胞、癌細胞>CRL-2547Panc 10.05 人胰腺腺癌細胞

Panc 10.05 人胰腺腺癌細胞

簡要描述:CRL-2547 Panc 10.05 人胰腺腺癌細胞,
原代細胞|細胞系|細胞株|菌種;細胞庫管理規(guī)范,
提供的細胞株背景清楚,提供參考文獻和培養(yǎng)條件!

  • 產(chǎn)品型號:CRL-2547
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-12
  • 訪  問  量:1892

產(chǎn)品分類

Product Category

詳細介紹

CRL-2547 Panc 10.05 人胰腺腺癌細胞

ATCC® Number: CRL-2547™ Price: $338.00

Designations: Panc 10.05

Depositors: EM Jaffee

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial

Source: Organ: pancreas

Disease: adenocarcinoma

Cellular Products: cytokeratins 7 and 18 [50655]

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


CRL-2547 Panc 10.05 人胰腺腺癌細胞

Isolation: Isolation date: 1992

Tumorigenic: Yes

Oncogene: K-ras +

Antigen Expression: MHC class I +; MHC class II -

DNA Profile (STR): Amelogenin: X

CSF1PO: 12

D13S317: 12

D16S539: 9,12

D5S818: 13

D7S820: 8,9

THO1: 6,9.3

TPOX: 11

vWA: 16

Age: adult

Gender: male

Ethnicity: White

Comments: Panc 10.05 is a pancreatic adenocarcinoma epithelial cell line derived in 1992 from a primary tumor removed from the head-of-the-pancreas of a male with pancreatic adenocarcinoma.

Both the PL45 and the Panc 10.05 cell lines exhibit a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. [50655]

The cells have a reported plating efficiency of 40%. [50655]

The Panc 10.05 cell line was derived from the same patient as the PL45 cell line (ATCC CRL-2558).

Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 10 Units/ml human insulin, 85%; fetal bovine serum, 15%

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 19.2 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

derived from same individual:ATCC CRL-2558

References: 50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602



CRL-2547
來自ATCC細胞,所有出入庫細胞均經(jīng)過嚴格的細胞質(zhì)量檢測,確保細胞無污染,符合檢測合格標準。歡迎來詢價詳談


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結果(填寫阿拉伯數(shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術支持:化工儀器網(wǎng)    管理登陸
伊人思思久99久女女精品视频| 欧洲美女与动性zozozo| 美女黄网站永久免费观看网站| 老师穿超短包臀裙办公室爆乳| 中文区中文字幕免费看| 亚洲精品第一国产综合野草社区| 资本主义生产关系| 奶涨边摸边做爰爽别停快点文 | 国产欧美日韩| 第123章 品尝孕妇滋味| 性的暴行中文完整版| 少妇挑战3个黑人叫声凄惨| 菠萝蜜视频免费观看| 国内老熟妇对白hdxxxx| 被夫の上司に犯中文字幕| 国产精品99久久久久久www| 奇米影视7777久久精品| 桃花免费高清在线观看视频| eeuss影院www在线观看| 我和亲妺妺乱的性视频| 色综合av综合无码综合网站| 免费无码又爽又刺激高潮视频| 噜妇插内射精品| 欧美日韩视频在线第一区| 爱的色放在线观看| 中文字幕爆乳巨爆乳系列| 永久免费精品精品永久-夜色 | 午夜无码乱码在线观看| 国产精品免费视频| 一出一进一爽一粗一大视频| 国产色综合久久无码有码| 人人爽久久久噜人人看| 成人免费a级毛片无码片2022| 高洁在公车被灌满jing液| 国产精品无码久久综合网| CHINESE老熟妇老女人HD| 99久久精品国产亚洲av| 门卫老李干了校花高小柔| 免费a级毛片在线播放不收费| 又黄又爽又猛1000部a片| 97SE亚洲国产综合自在线|